Francis Scott Key Bridge collapse in Baltimore...

I believe it was at 1:40am EST. But your right that had it been during the day possibly 100's would have been killed. From every video I have seen I can't find a shot that shows a car or truck on the bridge at the moment it collapsed.
The exact moment of impact (according to the timestamp on the harbor cam video) was 1:29am EDT this morning. The video clearly shows some semi trucks passing directly over the impact position within a minute (or so) of the bridge collapse. If the Mayday call shut down traffic to the bridge approach, it had to be immediately before or after the bridge was struck.