Yes. It’s a penalty if the ball doesn’t land in the landing zone
“In the NFL, a kickoff must travel to the area between the receiving team's goal line and their 20-yard line to land in the "landing zone," meaning any kick that lands within this zone must be returned by the receiving team; if the kick falls short of this area, it's treated as out of bounds and the ball is placed on the receiving team's 40-yard line”